cd64 apc cy7 Search Results


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Miltenyi Biotec cd64 apc cy7
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Thermo Fisher anti cd64 apc cy7
Co-expression of LEKTI attenuates matriptase-mediated premalignant skin phenotype. ( A ) The scheme shows the breeding of K5-LEKTI +/0 with K5-Matriptase +/0 mice and the resulting litter of WT, K5-LEKTI +/0 , K5-Matriptase +/0 , and K5-Matriptase +/0 /K5-LEKTI +/0 mice. Images show the outward appearance of these mice at 3 months of age. Matriptase-induced alopecia and ichthyosis are considerably attenuated by co-expression of LEKTI in Matriptase +/0 /K5-LEKTI +/0 . LEKTI +/0 in 3-month-old mice. ( B ) Representative histological appearance of dorsal skin of littermate WT (first column), K5-LEKTI +/0 (second column), K5-Matriptase +/0 (third column), and K5-Matriptase +/0 /K5-LEKTI +/0 mice (forth column) stained by H&E (top panels) and Toluidine Blue (bottom panels) at 3 months of age. Bars = 100 μm. Yellow dashed lines show the limits between epidermis and dermis. Black arrows show metachromatically stained dermal mast cells. ( C ) Quantification of epidermal thickness in littermate WT ( n = 7, black dots), K5-LEKTI +/0 ( n = 6, orange dots), K5-Matriptase +/0 ( n = 5, purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 ( n = 11, green dots) at 3 months of age. Data are expressed in mean ± SD. ( D ) Quantification of the dermal mast cell accumulation in the skin of littermate WT (black dots), K5-LEKTI +/0 (orange dots), K5-Matriptase +/0 (purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 (green dots) at 3 months of age. Data are expressed in mean ± SD. ( E – J ) Myeloid cellular infiltration was evaluated in skin samples by flow cytometry. ( E ) Total cells (×10 5 ) were assessed by automated cell counter using trypan blue. The percentage of ( F ) leukocytes (CD45 + ), ( G ) neutrophils (Ly6G + gated on CD45 + ), ( H ) dendritic cells (DC–CD11c + MHCII High gated on CD45 + Ly6G), ( I ) other myeloid cells (MY–CD11c + MHCII + gated on CD45 + Ly6G − ), and ( J ) macrophages <t>(CD64</t> + gated on myeloid cells) in the skin. WT (black dots), K5-LEKTI +/0 (orange dots), K5-Matriptase +/0 (purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 (green dots). ( K ) Representative flow cytometry of skin samples by groups. Data are representative of one experiment ( n = 3/group) and are expressed as means ± SD. p -values (One-Way ANOVA with Tukey’s post-hoc test) displayed in the graphs.
Anti Cd64 Apc Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher pe-cy7 cd11c n418
Antibody clones used to stain pulmonary mononuclear phagocytes
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Antibody clones used to stain pulmonary mononuclear phagocytes
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Biotium nk1.1 / cd161c / klrb1c, mouse(pk136)
Antibody clones used to stain pulmonary mononuclear phagocytes
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Becton Dickinson cd64-pe
Antibody clones used to stain pulmonary mononuclear phagocytes
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Thermo Fisher cd64 pe
Antibody clones used to stain pulmonary mononuclear phagocytes
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Bioss 15 lipoxygenase 1 polyclonal antibody, alexa fluor 647 conjugated
Antibody clones used to stain pulmonary mononuclear phagocytes
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Antibody clones used to stain pulmonary mononuclear phagocytes
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Antibody clones used to stain pulmonary mononuclear phagocytes
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Image Search Results


Co-expression of LEKTI attenuates matriptase-mediated premalignant skin phenotype. ( A ) The scheme shows the breeding of K5-LEKTI +/0 with K5-Matriptase +/0 mice and the resulting litter of WT, K5-LEKTI +/0 , K5-Matriptase +/0 , and K5-Matriptase +/0 /K5-LEKTI +/0 mice. Images show the outward appearance of these mice at 3 months of age. Matriptase-induced alopecia and ichthyosis are considerably attenuated by co-expression of LEKTI in Matriptase +/0 /K5-LEKTI +/0 . LEKTI +/0 in 3-month-old mice. ( B ) Representative histological appearance of dorsal skin of littermate WT (first column), K5-LEKTI +/0 (second column), K5-Matriptase +/0 (third column), and K5-Matriptase +/0 /K5-LEKTI +/0 mice (forth column) stained by H&E (top panels) and Toluidine Blue (bottom panels) at 3 months of age. Bars = 100 μm. Yellow dashed lines show the limits between epidermis and dermis. Black arrows show metachromatically stained dermal mast cells. ( C ) Quantification of epidermal thickness in littermate WT ( n = 7, black dots), K5-LEKTI +/0 ( n = 6, orange dots), K5-Matriptase +/0 ( n = 5, purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 ( n = 11, green dots) at 3 months of age. Data are expressed in mean ± SD. ( D ) Quantification of the dermal mast cell accumulation in the skin of littermate WT (black dots), K5-LEKTI +/0 (orange dots), K5-Matriptase +/0 (purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 (green dots) at 3 months of age. Data are expressed in mean ± SD. ( E – J ) Myeloid cellular infiltration was evaluated in skin samples by flow cytometry. ( E ) Total cells (×10 5 ) were assessed by automated cell counter using trypan blue. The percentage of ( F ) leukocytes (CD45 + ), ( G ) neutrophils (Ly6G + gated on CD45 + ), ( H ) dendritic cells (DC–CD11c + MHCII High gated on CD45 + Ly6G), ( I ) other myeloid cells (MY–CD11c + MHCII + gated on CD45 + Ly6G − ), and ( J ) macrophages (CD64 + gated on myeloid cells) in the skin. WT (black dots), K5-LEKTI +/0 (orange dots), K5-Matriptase +/0 (purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 (green dots). ( K ) Representative flow cytometry of skin samples by groups. Data are representative of one experiment ( n = 3/group) and are expressed as means ± SD. p -values (One-Way ANOVA with Tukey’s post-hoc test) displayed in the graphs.

Journal: Cancers

Article Title: Kallikrein 5 Inhibition by the Lympho-Epithelial Kazal-Type Related Inhibitor Hinders Matriptase-Dependent Carcinogenesis

doi: 10.3390/cancers13174395

Figure Lengend Snippet: Co-expression of LEKTI attenuates matriptase-mediated premalignant skin phenotype. ( A ) The scheme shows the breeding of K5-LEKTI +/0 with K5-Matriptase +/0 mice and the resulting litter of WT, K5-LEKTI +/0 , K5-Matriptase +/0 , and K5-Matriptase +/0 /K5-LEKTI +/0 mice. Images show the outward appearance of these mice at 3 months of age. Matriptase-induced alopecia and ichthyosis are considerably attenuated by co-expression of LEKTI in Matriptase +/0 /K5-LEKTI +/0 . LEKTI +/0 in 3-month-old mice. ( B ) Representative histological appearance of dorsal skin of littermate WT (first column), K5-LEKTI +/0 (second column), K5-Matriptase +/0 (third column), and K5-Matriptase +/0 /K5-LEKTI +/0 mice (forth column) stained by H&E (top panels) and Toluidine Blue (bottom panels) at 3 months of age. Bars = 100 μm. Yellow dashed lines show the limits between epidermis and dermis. Black arrows show metachromatically stained dermal mast cells. ( C ) Quantification of epidermal thickness in littermate WT ( n = 7, black dots), K5-LEKTI +/0 ( n = 6, orange dots), K5-Matriptase +/0 ( n = 5, purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 ( n = 11, green dots) at 3 months of age. Data are expressed in mean ± SD. ( D ) Quantification of the dermal mast cell accumulation in the skin of littermate WT (black dots), K5-LEKTI +/0 (orange dots), K5-Matriptase +/0 (purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 (green dots) at 3 months of age. Data are expressed in mean ± SD. ( E – J ) Myeloid cellular infiltration was evaluated in skin samples by flow cytometry. ( E ) Total cells (×10 5 ) were assessed by automated cell counter using trypan blue. The percentage of ( F ) leukocytes (CD45 + ), ( G ) neutrophils (Ly6G + gated on CD45 + ), ( H ) dendritic cells (DC–CD11c + MHCII High gated on CD45 + Ly6G), ( I ) other myeloid cells (MY–CD11c + MHCII + gated on CD45 + Ly6G − ), and ( J ) macrophages (CD64 + gated on myeloid cells) in the skin. WT (black dots), K5-LEKTI +/0 (orange dots), K5-Matriptase +/0 (purple dots), and K5-Matriptase +/0 /K5-LEKTI +/0 (green dots). ( K ) Representative flow cytometry of skin samples by groups. Data are representative of one experiment ( n = 3/group) and are expressed as means ± SD. p -values (One-Way ANOVA with Tukey’s post-hoc test) displayed in the graphs.

Article Snippet: The following conjugated primary antibodies were used for flow cytometry: anti-CD45-PeCy7 (clone 30-F11), anti-Ly6G-APC (clone IA8), anti-CD11c-FITC (clone HL3), anti-MHCII-BB700 (clone M5/114.15.2) from BD Bioscience (Franklin Lakes, NJ, USA), and anti-CD64-APC-Cy7 (clone X54-5/7.1) and anti-CD16/CD32 monoclonal antibody (clone 93) from eBioscience (San Diego, CA, USA).

Techniques: Expressing, Staining, Flow Cytometry

Antibody clones used to stain pulmonary mononuclear phagocytes

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes

doi: 10.1007/978-1-4939-8570-8_3

Figure Lengend Snippet: Antibody clones used to stain pulmonary mononuclear phagocytes

Article Snippet: Alternative fluorescent conjugates can also be used. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Clone Company Conjugate CD11c N418 eBioscience PE-Cy7 CD11b M1/70 eBioscience eF450 MHCII M5/114.15.2 eBioscience APC-Cy7 CD64 X54–5/7.1 BD Biosciences AF647 MerTK Polyclonal R&D Systems Unconjugated/biotin CD206 C06822 Biolegend PE Ly6C HK1.4 Biolegend BV510 P4B0 CLA3–1 AbDSerotec FITC Lyve-1 ALY7 eBioscience eF660 CD 169 SER-4 eBioscience PE CD36 No.72–1 eBioscience PE CCR2 475301 R&D Systems PE FcER1 42430 eBioscience FITC CD14 Sa2–8 eBioscience PerCP-Cy5.5 CD45 30-F11 eBioscience FITC CD45.1 A20 Biolegend PE-Cy7 CD45.2 104 Biolegend FITC Siglec F E50–2440 BD Biosciences PE/BV421 CD 103 2E7 eBioscience PE/eF450 BrdU Bu20A eBioscience APC CD43 S7 BD Biosciences BV421 CD24 M1/69 eBioscience eF450 CD115 AF598 Biolegend PE Ly6G 1A8 Biolegend eF450 B220 RA3–6B2 eBioscience eF450 CD3 17A2 eBioscience eF450 NK1.1 PK136 eBioscience eF450 Streptavidin eBioscience PerCP-Cy5.5 Open in a separate window Antibody clones used to stain pulmonary mononuclear phagocytes

Techniques: Clone Assay, Staining